Differentiating Allele Combinations of a Transgene Using Multiple Primer PCR
نویسندگان
چکیده
منابع مشابه
Primer Set Selection in Multiple PCR Experiments
The selection of a suitable set of primers is crucial to the multiple PCR (polymerase chain reaction) experiment, which is one of the most important techniques in molecular biology. The minimum primer set (MPS) problem is to minimize the number of primers required to amplify a set of DNA sequences, so that the experimental costs and time will be reduced. However, the MPS problem has been proved...
متن کاملOptimisation of PCR reactions using primer chessboarding.
In-house polymerase chain reaction (PCR) assays are now an integral part of the work of most diagnostic microbiological laboratories. Despite the availability of commercial reagent 'master-mixes' of some PCR reagents, the optimisation of primers still poses a significant problem. Here, we describe a simple method to assess the concentration of primer needed in single round, multiplex, nested an...
متن کاملBiologically-generated primer for PCR: PCR primer of unknown sequence
We describe a method for producing specific PCR primers directly from PCR product, bypassing the usual need to know the primer sequence. Lack of abundance of primers derived from a PCR product is compensated for by the incorporation of an arbitrary 5'TAG sequence which acts as a surrogate template target for the bulk amplification phase. We use the technique to amplify clonospecific rearranged ...
متن کاملMicroarray synthesis through multiple-use PCR primer design
A substantial percentage of the expense in constructing full-genome spotted microarrays comes from the cost of synthesizing the PCR primers to amplify the desired DNA. We propose a computationally-based method to substantially reduce this cost. Historically, PCR primers are designed so that each primer occurs uniquely in the genome. This condition is unnecessarily strong for selective amplifica...
متن کاملPCR Primer Design
To make PCR a specific, efficient and cost effective tool for researchers and clinicians the most important aspect is oligonucleotide primer design. This review discusses various aspects of primer design. Advice is provided for optimal design and the role of bioinformatic tools is highlighted. The authors discuss theoretical considerations and compare computational and experimental studies.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/96206bm02